ジャーナル
/
神経科学
/
Analysis of Trunk Neural Crest Cell Migration using a Modified Zigmond Chamber Assay
JoVE Journal
神経科学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 神経科学
Analysis of Trunk Neural Crest Cell Migration using a Modified Zigmond Chamber Assay

Analysis of Trunk Neural Crest Cell Migration using a Modified Zigmond Chamber Assay

12,338 Views

•

12:17 min

•

January 19, 2012

DOI:

10.3791/3330-v

DOI:
10.3791/3330-v

•

12:17 min
January 19, 2012

•

2 Views
, ,
1生物学科,California State University, Northridge, 2Centro de Biología Celular y Molecular,Universidad Nacional de Córdoba

概要

Automatically generated

An approach to analyze the migration of explanted cells (trunk neural crest cells) is described. This method is inexpensive, gentle, and capable of distinguishing chemotaxis from both chemokinesis and other influences on migratory polarity such as those derived from cell-cell interactions within the primary trunk neural crest cell culture.

Explore More Videos

Trunk Neural Crest Cell MigrationZigmond Chamber AssayEpithelial-mesenchymal TransitionTarget RecognitionNCC DifferentiationGuidance MechanismsNegative Guidance CuesChemoattractantsIn Vitro ApproachesPrimary Stem Cell Cultures

Read Article

Cite this Article

Walheim, C. C., Zanin, J. P., de Bellard, M. E. Analysis of Trunk Neural Crest Cell Migration using a Modified Zigmond Chamber Assay. J. Vis. Exp. (59), e3330, doi:10.3791/3330 (2012).

Download .ris file

Copy

Share Video

.

Copy