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1Department of Biomedical Engineering,University of Wisconsin-Madison, 2Materials Science Program,University of Wisconsin-Madison, 3Department of Neurological Surgery,University of Wisconsin-Madison, 4Carbone Comprehensive Cancer Center and Center for Stem Cell and Regenerative Medicine,University of Wisconsin-Madison
Chapters
- 00:05Title
- 01:57BTSC Neurosphere Dissociation
- 03:00Fabrication of Bi-layered su-8 Master
- 04:42Molding of the PDMS Stamp
- 05:31Assembly of Microfluidic Devices and Cell Culturing
- 07:04Long-term Time-lapse Imaging of BTSC Migration with BioStation IM
- 07:46Time-lapse Recordings of Migrating BTSC
- 08:40Conclusion
En compartmentalizing microfluidic enhet for etterforskning kreft stamceller migrasjon er beskrevet. Denne romanen plattform skapes en levedyktig mobilnettet mikromiljøet og gjør mikroskopisk visualisering av levende celle bevegelse. Svært bevegelige kreftceller er isolert for å studere molekylære mekanismer for aggressiv infiltrasjon, potensielt fører til mer effektive fremtidige terapier.
Tags
Cancer Stem Cell MigrationCompartmentalizing Microfluidic DevicesLive Cell ImagingCancer ResearchCellular MigrationAggressive Cancer Cell InvasionMetastasisTherapeutic ResistanceGlioblastoma Multiforme (GBM)Primary Malignant Adult Brain TumorGBM InfiltrationTumor MicroenvironmentCancer Cell Migration MechanismsBrain Tumor Stem Cell (BTSC)Therapeutic ResistanceRecurrenceCell Migratory MechanismsConventional Migration AssaysMicrofluidic DevicesSingle Cell Analysis
