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癌症干细胞迁移的使用进行划分的微流控芯片和活细胞成像的评价
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal Medicine
Evaluation of Cancer Stem Cell Migration Using Compartmentalizing Microfluidic Devices and Live Cell Imaging
DOI:

09:36 min

December 23, 2011

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Chapters

  • 00:05Title
  • 01:57BTSC Neurosphere Dissociation
  • 03:00Fabrication of Bi-layered su-8 Master
  • 04:42Molding of the PDMS Stamp
  • 05:31Assembly of Microfluidic Devices and Cell Culturing
  • 07:04Long-term Time-lapse Imaging of BTSC Migration with BioStation IM
  • 07:46Time-lapse Recordings of Migrating BTSC
  • 08:40Conclusion

Summary

Automatic Translation

一个调查癌症干细胞迁移的微流体装置进行划分。这个新的平台创建一个可行的细胞微环境,使活细胞运动的微观可视化。高度能动的癌症细胞分离分子机制研究的积极渗透,并可能导致未来更有效的疗法。

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