This content is Free Access.
57,428 Views
•
08:01 min
•
December 22, 2011
DOI:
Explore More Videos
VisualizingProteinsMacromolecular ComplexesNegative Stain EMGrid PreparationImage AcquisitionSingle Particle Electron MicroscopyStructural BiologyProtein StructuresCryoEMSample Preparation MethodDried Heavy Metal SaltSpecimen ContrastNegative Stain EMThree-dimensional StructurePurified ProteinsProtein ComplexesHomogeneity/heterogeneityLarge AssembliesQuality EvaluationEM ProtocolCarbon Coated GridsElectron Microscope
Read Article
Cite this Article
Booth, D. S., Avila-Sakar, A., Cheng, Y. Visualizing Proteins and Macromolecular Complexes by Negative Stain EM: from Grid Preparation to Image Acquisition. J. Vis. Exp. (58), e3227, doi:10.3791/3227 (2011).
Copy