Journal
/
Bioengineering
/
Visualisere Proteiner og makromolekylære Komplekser af negative Stain EM: fra Grid Forberedelse til Image Acquisition
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
Visualizing Proteins and Macromolecular Complexes by Negative Stain EM: from Grid Preparation to Image Acquisition

Visualisere Proteiner og makromolekylære Komplekser af negative Stain EM: fra Grid Forberedelse til Image Acquisition

English

Automatically Generated

57,428 Views

•

08:01 min

•

December 22, 2011

DOI:

10.3791/3227-v

DOI:
10.3791/3227-v

•

08:01 min
December 22, 2011

•

7 Views
, ,
1Graduate Group in Biophysics,University of California San Francisco , 2Department of Biochemistry and Biophysics,University of California San Francisco

Explore More Videos

VisualizingProteinsMacromolecular ComplexesNegative Stain EMGrid PreparationImage AcquisitionSingle Particle Electron MicroscopyStructural BiologyProtein StructuresCryoEMSample Preparation MethodDried Heavy Metal SaltSpecimen ContrastNegative Stain EMThree-dimensional StructurePurified ProteinsProtein ComplexesHomogeneity/heterogeneityLarge AssembliesQuality EvaluationEM ProtocolCarbon Coated GridsElectron Microscope

Read Article

Cite this Article

Booth, D. S., Avila-Sakar, A., Cheng, Y. Visualizing Proteins and Macromolecular Complexes by Negative Stain EM: from Grid Preparation to Image Acquisition. J. Vis. Exp. (58), e3227, doi:10.3791/3227 (2011).

Download .ris file

Copy

Share Video

.

Copy