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Flash-Photolyse von Caged Compounds in den Zilien der Riechzellen
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Neuroscience
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JoVE Journal Neuroscience
Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons
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Flash-Photolyse von Caged Compounds in den Zilien der Riechzellen

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12,690 Views

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11:35 min

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October 29, 2011

DOI:

10.3791/3195-v

DOI:
10.3791/3195-v

•

11:35 min
October 29, 2011

•

7 Views
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1SISSA,International School for Advanced Studies, 2Istituto di Biofisica,Consiglio Nazionale delle Ricerche, 3SISSA Unit,Italian Institute of Technology

Summary

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Photolyse von caged-Verbindungen ermöglicht die Produktion von schnellen und lokalisierten Anstieg der Konzentration von verschiedenen physiologisch aktiven Verbindungen. Hier zeigen wir, wie Patch-Clamp-Aufnahmen mit der Photolyse von caged cAMP kombiniert oder caged Ca für das Studium der olfaktorischen Wahrnehmung in dissoziierten Maus Riechzellen erhalten.

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Flash PhotolysisCaged CompoundsOlfactory Sensory NeuronsPatch-clamp RecordingsPhotolysis Of Caged CompoundsOlfactory TransductionDissociated Mouse Olfactory Sensory NeuronsCiliaOdorant BindingReceptorsCAMPCyclic Nucleotide-gated (CNG) ChannelsCa EntryCa-activated Cl ChannelsMouse Olfactory EpitheliumFlash LampUltraviolet FlashesWhole-cell Voltage-clamp Configuration

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Boccaccio, A., Sagheddu, C., Menini, A. Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons. J. Vis. Exp. (55), e3195, doi:10.3791/3195 (2011).

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