/
/
Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons
12,690 Views
•
11:35 min
•
October 29, 2011
DOI:
Summary
Automatically generated
Photolysis of caged compounds allows the production of rapid and localized increases in the concentration of various physiologically active compounds. Here, we show how to obtain patch-clamp recordings combined with photolysis of caged cAMP or caged Ca for the study of olfactory transduction in dissociated mouse olfactory sensory neurons.
Explore More Videos
Flash PhotolysisCaged CompoundsOlfactory Sensory NeuronsPatch-clamp RecordingsPhotolysis Of Caged CompoundsOlfactory TransductionDissociated Mouse Olfactory Sensory NeuronsCiliaOdorant BindingReceptorsCAMPCyclic Nucleotide-gated (CNG) ChannelsCa EntryCa-activated Cl ChannelsMouse Olfactory EpitheliumFlash LampUltraviolet FlashesWhole-cell Voltage-clamp Configuration
Read Article
Cite this Article
Boccaccio, A., Sagheddu, C., Menini, A. Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons. J. Vis. Exp. (55), e3195, doi:10.3791/3195 (2011).
Copy