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Voltaj-kapılı potasyum Kanal mRNA gösterilmeden Tek hücreli RT-PCR teknikleri kullanarak Nigral Nöronlar
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September 27, 2011
DOI:
Summary
Automatically generated
Nöronlar ilk elektrofizyolojik karakterizedir. Ardından kaydedilen nöron sitoplazma aspire ve nörotransmitter sentezi enzimler, iyon kanalları ve reseptörler mRNA'ların ifade algılamak için ters transkripsiyon-PCR analizi tabi.
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ProfilingVoltage-gated Potassium ChannelMRNA ExpressionNigral NeuronsSingle-cell RT-PCR TechniquesMammalian Central Nervous SystemNeuronsMolecular CharacteristicsFunctional CharacteristicsMorphologyGene Expression AnalysisSpecific Neuron TypeWhole-cell Patch Clamp Recording TechniquesSingle-cell Reverse Transcription Polymerase Chain Reaction (scRT-PCR)MRNA Expression ProfilingSubstantial NigraElectrophysiological TechniquesNeurophysiological PropertiesFunctional PropertiesCytoplasm AspirationScRT-PCR AnalysisNeurotransmitter Synthesis EnzymesReceptorsIon ChannelsSelectivitySensitivityImmunohistochemistryAntibody SuitabilityProtein Expression Level
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Ding, S., Zhou, F. M. Profiling Voltage-gated Potassium Channel mRNA Expression in Nigral Neurons using Single-cell RT-PCR Techniques. J. Vis. Exp. (55), e3136, doi:10.3791/3136 (2011).
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