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Perfiles de voltaje de expresión del ARNm de potasio en las neuronas Canal nigral con una sola celda de RT-PCR Técnicas
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September 27, 2011
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Summary
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Las neuronas se caracterizan primera electrofisiológica. A continuación, el citoplasma de las neuronas registradas se aspira y se somete a la transcripción reversa-PCR y análisis para detectar la expresión de ARNm para enzimas síntesis de neurotransmisores, canales iónicos y receptores.
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ProfilingVoltage-gated Potassium ChannelMRNA ExpressionNigral NeuronsSingle-cell RT-PCR TechniquesMammalian Central Nervous SystemNeuronsMolecular CharacteristicsFunctional CharacteristicsMorphologyGene Expression AnalysisSpecific Neuron TypeWhole-cell Patch Clamp Recording TechniquesSingle-cell Reverse Transcription Polymerase Chain Reaction (scRT-PCR)MRNA Expression ProfilingSubstantial NigraElectrophysiological TechniquesNeurophysiological PropertiesFunctional PropertiesCytoplasm AspirationScRT-PCR AnalysisNeurotransmitter Synthesis EnzymesReceptorsIon ChannelsSelectivitySensitivityImmunohistochemistryAntibody SuitabilityProtein Expression Level
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Ding, S., Zhou, F. M. Profiling Voltage-gated Potassium Channel mRNA Expression in Nigral Neurons using Single-cell RT-PCR Techniques. J. Vis. Exp. (55), e3136, doi:10.3791/3136 (2011).
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