A subscription to JoVE is required to view this content. Sign in or start your free trial.
13,709 Views
•
06:39 min
•
September 01, 2011
DOI:
1Temple University,Shriners Hospitals Pediatric Research Center and Department of Anatomy and Cell Biology, 2Medical Research Service,Department of Veterans Affairs Hospital, 3Department of Neurobiology and Anatomy,Drexel University College of Medicine, 4Shriners Hospitals Pediatric Research Center and Department of Anatomy and Cell Biology,Temple University School of Medicine
Summary
Automatically generated
Een<em> In vivo</em> Imaging-protocol om de primaire sensorische axonen volgende achterwortelganglia verpletteren monitor is beschreven. De procedures maken gebruik van wide-field fluorescentie microscopie en thy1-YFP transgene muizen, en staan herhaalde beeldvorming van regeneratie van axonen meer dan 4 cm in de PNS en axon interacties met de interface van het CZS.
Explore More Videos
Live ImagingDorsal Root AxonsRhizotomySpinal Root InjuriesChronic PainSensory LossRegenerationDorsal Root Entry Zone (DREZ)Molecular EventsCellular EventsNerve InjuryPostmortem AnalysesAxon RegenerationReal-time EventsLiving AnimalNeurons Serially In VivoOpticsMouse TransgenicsThy1-GFP Transgenic MiceFluorescent ColorsIn Vivo ImagingMuscleBrainPhysiological MechanismsStatic AnalysesSpinal Cord Imaging
Read Article
Cite this Article
Skuba, A., Himes, B. T., Son, Y. Live Imaging of Dorsal Root Axons after Rhizotomy. J. Vis. Exp. (55), e3126, doi:10.3791/3126 (2011).
Copy