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Live Imaging of Dorsal Root Axons after Rhizotomy
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06:39 min
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September 01, 2011
DOI:
1Temple University,Shriners Hospitals Pediatric Research Center and Department of Anatomy and Cell Biology, 2Medical Research Service,Department of Veterans Affairs Hospital, 3Department of Neurobiology and Anatomy,Drexel University College of Medicine, 4Shriners Hospitals Pediatric Research Center and Department of Anatomy and Cell Biology,Temple University School of Medicine
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An in vivo imaging protocol to monitor primary sensory axons following dorsal root crush is described. The procedures utilize wide-field fluorescence microscopy and thy1-YFP transgenic mice, and permit repeated imaging of axon regeneration over 4 cm in the PNS and axon interactions with the interface of the CNS.
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Cite this Article
Skuba, A., Himes, B. T., Son, Y. Live Imaging of Dorsal Root Axons after Rhizotomy. J. Vis. Exp. (55), e3126, doi:10.3791/3126 (2011).
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