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Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
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Biology
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Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

English

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42,531 Views

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10:21 min

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September 21, 2011

DOI:

10.3791/3060-v

DOI:
10.3791/3060-v

•

10:21 min
September 21, 2011

•

61 Views
, ,
1College of Nursing, Interdisciplinary Life Sciences Research Laboratory,Seattle University, 2College of Science and Engineering, Interdisciplinary Life Sciences Research Laboratory,Seattle University

Summary

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An automated method for identifying suitable hydrophobic interaction chromatography (HIC) media to be used in the process of protein purification is presented. The method utilizes a medium-pressure liquid chromatography system including automated buffer blending, dynamic sample loop injection, sequential column selection, multi-wavelength analysis, and split fraction eluate collection.

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Cite this Article

Murphy, P. J. M., Stone, O. J., Anderson, M. E. Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification. J. Vis. Exp. (55), e3060, doi:10.3791/3060 (2011).

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