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Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
Kapitel
- 00:05Titel
- 01:30Buffer and Sample Preparation
- 02:48Physical Setup and Plumbing of the DuoFlow Chromatography System
- 04:11Priming System Lines,Programming Run Method, and Equilibrating HIC Columns
- 05:43Sample Loading, Programming the Software, and Running the Column Scouting Protocol
- 07:36Representative Chromatogram and Fluorescent Fraction Data
- 09:23Conclusion
An automated method for identifying suitable hydrophobic interaction chromatography (HIC) media to be used in the process of protein purification is presented. The method utilizes a medium-pressure liquid chromatography system including automated buffer blending, dynamic sample loop injection, sequential column selection, multi-wavelength analysis, and split fraction eluate collection.
