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Kombine Kullanımı Hedefleme Koku Ampul Nöronlar In Vivo Elektroporasyon ve Gal4 Tabanlı Enhancer Trap Zebra balığı Hatları
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines
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Kombine Kullanımı Hedefleme Koku Ampul Nöronlar In Vivo Elektroporasyon ve Gal4 Tabanlı Enhancer Trap Zebra balığı Hatları

Türkçe

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11,850 Views

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08:18 min

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August 15, 2011

DOI:

10.3791/2964-v

DOI:
10.3791/2964-v

•

08:18 min
August 15, 2011

•

1 Views
, , ,
1Department of Biology,Pace University, 2Cellular and Molecular Medicine,University of California, San Diego, 3Division of Cell Biology and Cell Physiology, Zoological Institute,Braunschweig University of Technology

Summary

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Genetik manipülasyon zamansal ve mekansal çözünürlükte karıştırmayı ki biyolojik olayların spektrum belirler. Burada zamansal ve mekansal ayrık<em> In vivo</emZebrafish transgenik hatları ile birlikte, özellikle gelişmekte olan koku alma ampul nöronlarda GFP transgen ifadesi neden> elektroporasyon,.

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Hoegler, K. J., Distel, M., Köster, R. W., Horne, J. H. Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines. J. Vis. Exp. (54), e2964, doi:10.3791/2964 (2011).

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