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Transplante de Células-Tronco cirúrgica do rato Neural na medula espinhal de camundongos infectados com o vírus da Hepatite neurotrófico rato
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July 10, 2011
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O transplante de células-tronco do mouse neural (NSCs) na medula espinhal de ratos com desmielinização estabelecida é detalhado. A preparação de NSCs, a laminectomia de vértebra torácica 9 (T9), e transplante de NSCs é descrito juntamente com os cuidados pré e pós-operatório dos ratos.
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Surgical TransplantationMouse Neural Stem CellsSpinal CordsMice InfectedNeurotropic Mouse Hepatitis VirusJHM StrainDemyelinating DiseaseMultiple SclerosisTransplantation Of NSCsRemyelinationClinical OutcomeCell Replacement TherapiesChronic Neurologic DiseasesIn Vivo ModelsEngrafted CellsAdapted MethodI) Preparation Of NSCsIi) Pre-operative Care Of MiceIii) Exposure Of The Spinal Cord Via LaminectomyIv) Stereotactic Injection Of NSCsIv) Post-operative Care
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Carbajal, K. S., Weinger, J. G., Whitman, L. M., Schaumburg, C. S., Lane, T. E. Surgical Transplantation of Mouse Neural Stem Cells into the Spinal Cords of Mice Infected with Neurotropic Mouse Hepatitis Virus. J. Vis. Exp. (53), e2834, doi:10.3791/2834 (2011).
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