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Métodos para avaliar Mediated Beta Morte celular por linfócitos T citotóxicos
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Immunology and Infection
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JoVE Journal Immunology and Infection
Methods to Assess Beta Cell Death Mediated by Cytotoxic T Lymphocytes

Métodos para avaliar Mediated Beta Morte celular por linfócitos T citotóxicos

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13,256 Views

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12:12 min

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June 16, 2011

DOI:

10.3791/2724-v

DOI:
10.3791/2724-v

•

12:12 min
June 16, 2011

•

5 Views
, ,
1Departments of Pathology, Immunology and Laboratory Medicine, College of Medicine,University of Florida

Summary

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Mediada por células-linfocitotoxicidade (CML) os ensaios podem ser usados ​​para testar as respostas auto-reativos e mecanismos de estudo de morte celular in vitro. No entanto, usando live-célula confocal técnicas de imagem microscópica com corantes fluorescentes, do tipo e da cinética de morte celular, bem como as vias utilizadas podem ser estudados em maior detalhe.

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Cite this Article

Chen, J., Grieshaber, S., Mathews, C. E. Methods to Assess Beta Cell Death Mediated by Cytotoxic T Lymphocytes. J. Vis. Exp. (52), e2724, doi:10.3791/2724 (2011).

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