Journal
/
Neuroscience
/
Neurais Colony-Forming Assay Cell: um ensaio para Discriminar Bona Fide células-tronco neurais de células progenitoras neurais
JoVE Journal
Neuroscience
Author Produced
This content is Free Access.
JoVE Journal Neuroscience
Neural-Colony Forming Cell Assay: An Assay To Discriminate Bona Fide Neural Stem Cells from Neural Progenitor Cells

Neurais Colony-Forming Assay Cell: um ensaio para Discriminar Bona Fide células-tronco neurais de células progenitoras neurais

português

Automatically Generated

21,780 Views

•

06:31 min

•

March 06, 2011

DOI:

10.3791/2639-v

DOI:
10.3791/2639-v

•

06:31 min
March 06, 2011

•

3 Views
, , , ,
1Department of Neurosurgery,University of Florida, 2Department of Anatomical Sciences,Shiraz University of Medical Sciences, 3STEMCELL Technologies,Inc.

Summary

Automatically generated

Este protocolo vídeo demonstra como discriminar e enumerar bona fide células-tronco neurais em uma população mista de células precursoras neurais utilizando o ensaio de célula neural formadoras de colônias.

Explore More Videos

Neural-Colony Forming Cell AssayAssayDiscriminateNeural Stem CellsNeural Progenitor CellsNeurosphere AssayIsolateExpandFrequencySerum-free Culture SystemTumorsNeurosphere FormationOverestimatesMixed PopulationEmbryonic BrainAdult Mammalian BrainCollagen-based Semi-solid AssayN-CFCAProliferative PotentialEnumerate NSC FrequencyColoniesFunctional CriteriaPrimary CellsCultured Cells

Read Article

Cite this Article

Azari, H., Louis, S. A., Sharififar, S., Vedam-Mai, V., Reynolds, B. A. Neural-Colony Forming Cell Assay: An Assay To Discriminate Bona Fide Neural Stem Cells from Neural Progenitor Cells. J. Vis. Exp. (49), e2639, doi:10.3791/2639 (2011).

Download .ris file

Copy

Share Video

.

Copy