This video protocol demonstrates how to discriminate and enumerate bona fide neural stem cells in a mixed population of neural precursor cells using the neural colony-forming cell assay.
Azari, H., Louis, S. A., Sharififar, S., Vedam-Mai, V., Reynolds, B. A. Neural-Colony Forming Cell Assay: An Assay To Discriminate Bona Fide Neural Stem Cells from Neural Progenitor Cells. J. Vis. Exp. (49), e2639, doi:10.3791/2639 (2011).