Journal
/
Biology
/
Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death

Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death

English

Automatically Generated

101,428 Views

•

07:34 min

•

April 24, 2011

DOI:

10.3791/2597-v

DOI:
10.3791/2597-v

•

07:34 min
April 24, 2011

•

86 Views
, , ,
1Department of Biological Sciences,University of Alberta, 2Department of Agriculture, Food and Nutrition Sciences,University of Alberta

Summary

Automatically generated

An accurate method for the assessment of cell death is described. The protocol improves upon conventional Annexin V/ propidium iodide (PI) protocols, which display up to 40% false- positive events in cell lines and primary cells from a broad range of animal models.

Explore More Videos

Annexin V/Propidium Iodide Apoptosis AssayCell Death StudiesFlow Cytometry-based MethodsPlasma Membrane IntegrityPermeabilityCell ViabilityNuclear StainsLate Apoptotic CellsNecrotic CellsFalse Positive EventsRNA StainingCytoplasmic Compartment

Read Article

Cite this Article

Rieger, A. M., Nelson, K. L., Konowalchuk, J. D., Barreda, D. R. Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death. J. Vis. Exp. (50), e2597, doi:10.3791/2597 (2011).

Download .ris file

Copy

Share Video

.

Copy