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Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death
JoVE Journal
Biologia
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JoVE Journal Biologia
Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death

Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death

101,428 Views

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07:34 min

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April 24, 2011

DOI:

10.3791/2597-v

DOI:
10.3791/2597-v

•

07:34 min
April 24, 2011

•

86 Views
, , ,
1Department of Biological Sciences,University of Alberta, 2Department of Agriculture, Food and Nutrition Sciences,University of Alberta

Summary

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An accurate method for the assessment of cell death is described. The protocol improves upon conventional Annexin V/ propidium iodide (PI) protocols, which display up to 40% false- positive events in cell lines and primary cells from a broad range of animal models.

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Rieger, A. M., Nelson, K. L., Konowalchuk, J. D., Barreda, D. R. Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death. J. Vis. Exp. (50), e2597, doi:10.3791/2597 (2011).

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