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Immunology and Infection
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Using Bioluminescent Imaging to Investigate Synergism Between Streptococcus pneumoniae and Influenza A Virus in Infant Mice
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Using Bioluminescent Imaging to Investigate Synergism Between Streptococcus pneumoniae and Influenza A Virus in Infant Mice

Using Bioluminescent Imaging to Investigate Synergism Between Streptococcus pneumoniae and Influenza A Virus in Infant Mice

DOI:
10.3791/2357-v

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10:47 min

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April 14, 2011

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, , , , , ,
1Department of Microbiology and Immunology,University of Melbourne, 2Laboratory of Pediatric Infectious Diseases,Radboud University Nijmegen Medical Centre, 3The Centre for Dynamic Imaging,The Walter and Eliza Hall Institute for Medical Research

Chapters

  • 00:05Title
  • 01:47Preparation of Infectious Stock of Bioluminescent S. pneumoniae
  • 03:14Growth and Preparation of Influenza A Virus Stocks
  • 06:22Intranasal Infection of Mice with Bioluminescent S pneumoniae and/or Influenza A Virus
  • 07:25Bioluminescent Imaging Using the IVIS Spectrum (Caliper Sciences)
  • 09:15In vivo Bioluminescent Imaging of Co-Infection of Respiratory Tract with S. pneumoniae and Influenza Virus
  • 10:21Conclusion

Summary

Automatic Translation

Automatic Translation

A concurrent infection with influenza A virus is one of the factors implicated in the induction of invasive pneumococcal disease during asymptomatic Streptococcus pneumoniae carriage. Here we describe a mixed infection method using infant mice to investigate the synergism between these two respiratory pathogens.

Tags

Bioluminescent ImagingSynergismStreptococcus PneumoniaeInfluenza A VirusInfant Mice1918 Influenza PandemicSecondary Bacterial InfectionPneumococcus1957 Asian Influenza PandemicSeasonal OutbreaksMorbidityConcurrent InfectionMurine ModelInfluenza Virus InfectionS. Pneumoniae ColonizationKineticsIn Vivo Imaging

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