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Monitoring Immune Cells Trafficking Fluorescent Prion Rods Hours after Intraperitoneal Infection
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November 19, 2010
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Here we describe a novel assay for monitoring prion uptake and trafficking by immune cells immediately following intraperitoneal inoculation by purifying and fluorescently labeling aggregated prion rods from infected brain material then monitoring their uptake and movement from the injection site and characterizing the cells mediating these events.
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Immune CellsTraffickingFluorescent Prion RodsIntraperitoneal InfectionAbnormal FormHost-encoded Prion Protein (PrPC)Protease ResistantPathologicInfectiousPrion DiseasesChronic Wasting Disease (CWD)ScrapiePrion HypothesisImmune SystemPeripheral Prion PathogenesisComplement SystemDendritic CellsMacrophagesPrion UptakeTraffickingInoculation Site
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Johnson, T. E., Michel, B. A., Meyerett, C., Duffy, A., Avery, A., Dow, S., Zabel, M. D. Monitoring Immune Cells Trafficking Fluorescent Prion Rods Hours after Intraperitoneal Infection. J. Vis. Exp. (45), e2349, doi:10.3791/2349 (2010).
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