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Immunology and Infection

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JoVE Journal Immunology and Infection
Monitoring Immune Cells Trafficking Fluorescent Prion Rods Hours after Intraperitoneal Infection
 

Monitoring Immune Cells Trafficking Fluorescent Prion Rods Hours after Intraperitoneal Infection

Article DOI: 10.3791/2349-v • 09:22 min • November 19th, 2010
November 19th, 2010 •
*1, *1, 1, 1, 1, 1, 1
1Department of Microbiology, Immunology and Pathology, Colorado State University
* These authors contributed equally

Kapitel

Summary
Automatic Translation

Here we describe a novel assay for monitoring prion uptake and trafficking by immune cells immediately following intraperitoneal inoculation by purifying and fluorescently labeling aggregated prion rods from infected brain material then monitoring their uptake and movement from the injection site and characterizing the cells mediating these events.

Tags

Immune Cells Trafficking Fluorescent Prion Rods Intraperitoneal Infection Abnormal Form Host-encoded Prion Protein (PrPC) Protease Resistant Pathologic Infectious Prion Diseases Chronic Wasting Disease (CWD) Scrapie Prion Hypothesis Immune System Peripheral Prion Pathogenesis Complement System Dendritic Cells Macrophages Prion Uptake Trafficking Inoculation Site
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