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粘着テープベースのサンプリングと蛍光の組み合わせその場でハイブリダイゼーションサルモネラ生鮮食品について
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Immunology and Infection
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JoVE Journal Immunology and Infection
Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
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粘着テープベースのサンプリングと蛍光の組み合わせその場でハイブリダイゼーションサルモネラ生鮮食品について

日本語

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12,818 Views

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09:10 min

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October 18, 2010

DOI:

10.3791/2308-v

DOI:
10.3791/2308-v

•

09:10 min
October 18, 2010

•

6 Views
,
1Center for Meat Safety and Quality, Department of Animal Sciences,Colorado State University, 2Rapid Microbial Detection and Control Laboratory, Department of Food Science and Human Nutrition,Iowa State University

Summary

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このプロトコルは、急速な細胞全体の検出に続いてトマトや他の生鮮食品の表面のサンプリングのための簡単​​な粘着テープベースのアプローチを、説明して<em>サルモネラ</em>蛍光を使用して<em>その場で</em>ハイブリダイゼーション（FISH）。

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Adhesive-tape-based SamplingFluorescence In Situ HybridizationRapid DetectionSalmonellaFresh ProduceCulture-independent DetectionCell-charged TapesSolid-phase EnrichmentLiquid Surface MinicultureDNA Probe CocktailHybridizationFluorescence Microscopy

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Bisha, B., Brehm-Stecher, B. F. Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce. J. Vis. Exp. (44), e2308, doi:10.3791/2308 (2010).

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