Journal
/
Imunologia e Infecção
/
Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce

Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce

12,818 Views

•

09:10 min

•

October 18, 2010

DOI:

10.3791/2308-v

DOI:
10.3791/2308-v

•

09:10 min
October 18, 2010

•

6 Views
,
1Center for Meat Safety and Quality, Department of Animal Sciences,Colorado State University, 2Rapid Microbial Detection and Control Laboratory, Department of Food Science and Human Nutrition,Iowa State University

Summary

Automatically generated

This protocol describes a simple adhesive-tape-based approach for sampling of tomato and other fresh produce surfaces, followed by rapid whole cell detection of Salmonella using fluorescence in situ hybridization (FISH).

Explore More Videos

Adhesive-tape-based SamplingFluorescence In Situ HybridizationRapid DetectionSalmonellaFresh ProduceCulture-independent DetectionCell-charged TapesSolid-phase EnrichmentLiquid Surface MinicultureDNA Probe CocktailHybridizationFluorescence Microscopy

Read Article

Cite this Article

Bisha, B., Brehm-Stecher, B. F. Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce. J. Vis. Exp. (44), e2308, doi:10.3791/2308 (2010).

Download .ris file

Copy

Share Video

.

Copy