JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
Retroviral Transduction of T-cell Receptors in Mouse T-cells
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Retroviral Transduction of T-cell Receptors in Mouse T-cells
Retroviral Transduction of T-cell Receptors in Mouse T-cells
DOI:
10.3791/2307-v
•
10:14 min
•
October 22, 2010
•
Shi Zhong
,
Karolina Malecek
2
,
Arianne Perez-Garcia
,
Michelle Krogsgaard
1
NYU Cancer institute
,
New York University School of Medicine
,
2
Program in Structural Biology
,
New York University School of Medicine
Chapters
00:00
Title
01:37
Retroviral Transfection and T-cell Isolation
03:55
Prepapration of a Single-cell Suspension from Mouse Spleen
04:51
Isolation and Activation of Mouse T-cells
06:27
Infection of Activated T-cells
08:21
Expansion of Cells and Evaluation of Transduction Efficiency
08:55
Retroviral Expression of TCR on Mouse T-cells
09:32
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
We present a protocol to produce antigen-specific mouse T-cells using retroviral transduction
Tags
Retroviral Transduction
T-cell Receptors
Mouse T-cells
Immune System
Peptide Antigens
Antigen Presenting Cells
Functional Outcomes
Immunology Related Diseases
TCR Transgenic Mouse Models
Bone Marrow Chimera
Retroviral Transduction Method
Peptide-MHC Specificity
Downstream Applications
Human Immunotherapy
Adoptive Transfer
Cancer Treatment
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
check_url/2307?article_type=v
Generering av Utførte regulatoriske T-celler fra primære humane naiv og Minne T-celler
check_url/2307?article_type=v
piggyBac
transposon System Endring av primære humane T celler
check_url/2307?article_type=v
Adenoviral Transduksjon av Naive CD4 T-celler for å studere Treg Differensiering
check_url/2307?article_type=v
Avledning av T-celler<em> In Vitro</em> Fra Mouse Embryonic Stem Cells
check_url/2307?article_type=v
Mus naive CD4<sup> +</sup> T Cell Isolasjon og<em> In vitro</em> Differensiering i T cell Subsets
check_url/2307?article_type=v
Retroviral Transduksjon av stamceller i benmarg for å generere T-celle reseptor Retrogenic Mus
check_url/2307?article_type=v
Generering<em> De Novo</em> Antigen-spesifikke humane T-cellereseptorer etter retroviral transduksjon av Centric Hemichain
check_url/2307?article_type=v
Retroviral Transduksjon T-hjelpeceller som genetisk tilnærming å studere mekanismer kontrollere sine Differensiering og funksjon
check_url/2307?article_type=v
In vivo styrking av gut-homing regulatoriske T Cell induksjon
check_url/2307?article_type=v
Isolering av mus nyre-resident CD8
+
T celler for Flow Cytometry Analyse
Read Article