Journal
/
神经科学
/
Optic Nerve Transection: A Model of Adult Neuron Apoptosis in the Central Nervous System
JoVE 杂志
神经科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
Optic Nerve Transection: A Model of Adult Neuron Apoptosis in the Central Nervous System

Optic Nerve Transection: A Model of Adult Neuron Apoptosis in the Central Nervous System

DOI:
10.3791/2241-v

•

12:06 min

•

May 12, 2011

•

, ,
1Department of Surgery,University of Toronto

Chapters

  • 00:05Title
  • 01:22Surgical Approach
  • 05:59Accessing the Optic Nerve
  • 08:31Closing and Recovery
  • 09:35Representative Results
  • 11:27Conclusion

Summary

自动翻译

自动翻译

Optic Nerve transection is a widely used model of adult CNS injury. Ninety percent of retinal ganglion cells (RGCs) whose axons are completely transected (axotomy) die within 14 days after axotomy. This model is easily amenable to experimental manipulations and highly reproducible.

Tags

Optic Nerve TransectionAdult Neuron ApoptosisCentral Nervous SystemRetinal Ganglion CellsOptic NerveAxotomizedApoptotic Neuronal Cell DeathCNS ModelVitreous ChamberDrug DeliveryIntraocular InjectionsShort Interfering RNAs (siRNAs)PlasmidsViral VectorsSuperior ColliculusApoptotic MechanismsNeuronal PopulationCell Survival QuantificationFluorescent Tracer

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/2241?article_type=v

Methods for Experimental Manipulations after Optic Nerve Transection in the Mammalian CNS
check_url/cn/2241?article_type=v

Multimodal Imaging of Stem Cell Implantation in the Central Nervous System of Mice
check_url/cn/2241?article_type=v

Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System
check_url/cn/2241?article_type=v

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster
check_url/cn/2241?article_type=v

An Injury Paradigm to Investigate Central Nervous System Repair in Drosophila
check_url/cn/2241?article_type=v

Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System
check_url/cn/2241?article_type=v

In vivo Interrogation of Central Nervous System Translatome by Polyribosome Fractionation
check_url/cn/2241?article_type=v

In vivo Optogenetic Stimulation of the Rodent Central Nervous System
check_url/cn/2241?article_type=v

Visual Evoked Potential Recording in a Rat Model of Experimental Optic Nerve Demyelination
check_url/cn/2241?article_type=v

Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY

Read Article