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July 15, 2010
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El siguiente protocolo proporciona instrucciones para la adaptación humana células madre pluripotentes inducidas (iPS) celdas para alimentación libre de la cultura a través del alimentador de Libre completa golpe de gracia suero sustitución medio (KSR-FF). Una vez adaptado, instrucciones para el mantenimiento continuo también se proporcionan.
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Feeder-Free AdaptationCulturePassagingHuman IPS CellsComplete KnockOut Serum ReplacementFeeder-Free MediumReprogrammedIPS CellsEmbryonic Stem CellsPluripotent CellsDrug DiscoveryCell TherapyBasic ResearchGIBCO Media And ReagentsKnockout DMEMKnockout Serum ReplacementIPS Cell CultureFeeder-free CultureKnockout SR Growth Factor CocktailTraditional Culture MethodsMouse Fibroblast Feeder LayersHuman Fibroblast Feeder LayersFeeder-conditioned MediumLabor-intensiveHard To ScaleUndifferentiated HiPS CellsUndefined Conditions
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Wagner, K., Welch, D. Feeder-Free Adaptation, Culture and Passaging of Human IPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium. J. Vis. Exp. (41), e2236, doi:10.3791/2236 (2010).
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