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Feeder-Free Adaptation, Culture and Passaging of Human IPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium
Journal JoVE
Biologie
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Journal JoVE Biologie
Feeder-Free Adaptation, Culture and Passaging of Human IPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium

Feeder-Free Adaptation, Culture and Passaging of Human IPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium

DOI:
10.3791/2236-v

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08:29 min

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July 15, 2010

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1GIBCO,Life Technologies

Chapitres

  • 00:04Titre
  • 00:45Introduction
  • 01:33Preparing Geltrex-coated Culture Dishes
  • 02:59Adapting Human iPSCs
  • 05:20Passaging Human iPSCs
  • 07:36Representative Results
  • 08:07Conclusion

Summary

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The following protocol provides instruction for adapting human induced Pluripotent Stem (iPS) Cells to feeder-free culture using complete KnockOut Serum Replacement Feeder-Free medium (KSR-FF). Once adapted, instructions for continual maintenance are also provided.

Tags

Feeder-Free AdaptationCulturePassagingHuman IPS CellsComplete KnockOut Serum ReplacementFeeder-Free MediumReprogrammedIPS CellsEmbryonic Stem CellsPluripotent CellsDrug DiscoveryCell TherapyBasic ResearchGIBCO Media And ReagentsKnockout DMEMKnockout Serum ReplacementIPS Cell CultureFeeder-free CultureKnockout SR Growth Factor CocktailTraditional Culture MethodsMouse Fibroblast Feeder LayersHuman Fibroblast Feeder LayersFeeder-conditioned MediumLabor-intensiveHard To ScaleUndifferentiated HiPS CellsUndefined Conditions

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