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July 15, 2010
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以下协议提供馈线自由的文化，使用完整的基因敲除血清替代馈线培养基（KSR - FF）为适应人类诱导多能干细胞（iPS）细胞的指令。一旦适应，也提供了不断维护的说明。
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Feeder-Free AdaptationCulturePassagingHuman IPS CellsComplete KnockOut Serum ReplacementFeeder-Free MediumReprogrammedIPS CellsEmbryonic Stem CellsPluripotent CellsDrug DiscoveryCell TherapyBasic ResearchGIBCO Media And ReagentsKnockout DMEMKnockout Serum ReplacementIPS Cell CultureFeeder-free CultureKnockout SR Growth Factor CocktailTraditional Culture MethodsMouse Fibroblast Feeder LayersHuman Fibroblast Feeder LayersFeeder-conditioned MediumLabor-intensiveHard To ScaleUndifferentiated HiPS CellsUndefined Conditions
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Wagner, K., Welch, D. Feeder-Free Adaptation, Culture and Passaging of Human IPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium. J. Vis. Exp. (41), e2236, doi:10.3791/2236 (2010).
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