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Génération d'ADN / ARN hybrides d'ADN génomique par la transformation en utilisant l'ARN contenant Oligonucléotides
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides
DOI:

16:42 min

November 24, 2010

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Chapters

  • 00:05Title
  • 01:20Introduction
  • 01:53Preparation of the RNA-containing Oligo and the Yeast Cells for Transformation
  • 04:40Transformation of Yeast Cells Using the RNA-Containing Oligo
  • 07:47Analysis of Gene Correction by the RNA-Containing Oligo
  • 11:10Alkali Treatment of the RNA-Containing Oligo
  • 12:20Representative Results for Transformation Using RNA-Containing Oligonucleotides
  • 16:10Conclusion

Summary

Automatic Translation

Ce travail montre comment former un hybride ARN / ADN au niveau chromosomique et de révéler le transfert de l'information génétique à partir d'ARN à l'ADN génomique dans les cellules de levure.

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