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Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides
Kapitel
- 00:05Titel
- 01:20Introduction
- 01:53Preparation of the RNA-containing Oligo and the Yeast Cells for Transformation
- 04:40Transformation of Yeast Cells Using the RNA-Containing Oligo
- 07:47Analysis of Gene Correction by the RNA-Containing Oligo
- 11:10Alkali Treatment of the RNA-Containing Oligo
- 12:20Representative Results for Transformation Using RNA-Containing Oligonucleotides
- 16:10Conclusion
Tags
RNA/DNA HybridsGenomic DNATransformationRNA-containing OligonucleotidesSynthetic Short Nucleic Acid PolymersOligonucleotidesMolecular Biology ToolsBase And Sugar ModificationsNucleic Acid AlterationsDNA DamageMechanisms Of RepairThermo Scientific Dharmacon RNA-containing OligosIn Vivo StudyChromosomal RNA/DNA HybridsRibonucleotides Embedded Into DNAGenetic Integrity Of CellsRibonucleotide IncorporationEukaryotic Model System Yeast/Saccharomyces Cerevisiae
