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Genomweite Analyse mittels ChIP zu Isoform-spezifische Gene-Targets zu identifizieren
JoVE Journal
Biology
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JoVE Journal Biology
Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets
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Genomweite Analyse mittels ChIP zu Isoform-spezifische Gene-Targets zu identifizieren

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14,200 Views

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11:19 min

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July 07, 2010

DOI:

10.3791/2101-v

DOI:
10.3791/2101-v

•

11:19 min
July 07, 2010

•

1 Views
, , , , , ,
1Department of Biochemistry and Molecular Genetics,University of Illinois Chicago – UIC, 2Research Unit on Biomedical Informatics,Universitat Pompeu Fabra, 3Genome Technology Core,Whitehead Institute for Biomedical Research

Summary

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Hier präsentieren wir eine Chromatin-Immunopräzipitation (ChIP) Verfahren zur genomweiten Ort Analyse von Protein-Isoformen, die in einer Histon-bindende Domäne unterscheiden. Wir wenden es ChIP-Seq-Analyse, um die Ziele des KDM5A/JARID1A/RBP2 Histon-Demethylase zu identifizieren.

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Beshiri, M. L., Islam, A., DeWaal, D. C., Richter, W. F., Love, J., Lopez-Bigas, N., Benevolenskaya, E. V. Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets. J. Vis. Exp. (41), e2101, doi:10.3791/2101 (2010).

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