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Transurethral Induction of Mouse Urinary Tract Infection
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JoVE Journal İmmünoloji ve Enfeksiyon
Transurethral Induction of Mouse Urinary Tract Infection

Transurethral Induction of Mouse Urinary Tract Infection

DOI:
10.3791/2070-v

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09:24 min

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August 05, 2010

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, ,
1Earth Systems Program, School of Earth Sciences,Stanford University , 2Department of Urology,Stanford University School of Medicine

Bölümler

  • 00:04Başlık
  • 00:43Introduction
  • 01:10Preparation of Urethral Catheters
  • 01:59Bacterial Growth and Animal Inoculation
  • 05:49Measuring CFU in Infected Tissue
  • 08:34Representative Results
  • 08:55Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

This video will demonstrate methods to transurethrally induce mouse urinary tract infections and quantify the extent of resulting infections.

Etiketler

Transurethral InductionMouse Urinary Tract InfectionUropathogenic Bacterial StrainsAgarUropathogenic E. ColiLuria-Bertani AgarColony MorphologyCulture In BrothLB BrothType I PiliVirulence FactorPhosphate Buffered SalineFemale MiceIsoflurane AnesthesiaTransurethral InoculationPolyethylene Tubing-covered SyringesInocula ConcentrationCfu Per MouseUrine SamplesBladderKidneysHarvesting OrgansMinced OrgansHomogenizationBlue Bullet HomogenizerSerial DilutionColony Forming Units

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