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Visualizando transferência de células-célula de HIV usando Clones fluorescente de HIV e Microscopia Confocal ao vivo
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October 07, 2010
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Este experimento visualizado é um guia para a utilização de um clone fluorescentes molecular do HIV para viver experiências imagem confocal.
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VisualizingCell-to-cell TransferHIVFluorescent ClonesLive Confocal MicroscopyGreen Fluorescent ProteinComplex Cellular ProcessesFluorescence Video MicroscopyHuman Immunodeficiency Virus Core ProteinGag ProteinInfectious Molecular CloneHIV Gag-iGFPVideo Confocal MicroscopyTransfectHuman T Cell LineFluorescently Labeled Uninfected CD4+ T CellsTarget CellsViral ProductionTransportIntercellular StructuresVirological SynapsesGas Permeable Imaging Chambers
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Dale, B., McNerney, G. P., Thompson, D. L., Hübner, W., Huser, T., Chen, B. K. Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy. J. Vis. Exp. (44), e2061, doi:10.3791/2061 (2010).
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