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Visualizzazione Cell-per-cell trasferimento del virus HIV usando cloni fluorescenti di HIV e Live Microscopia confocale
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October 07, 2010
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Summary
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Questo esperimento viene visualizzata una guida per l'utilizzo fluorescente un clone molecolare di HIV per vivere esperimenti di imaging confocale.
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VisualizingCell-to-cell TransferHIVFluorescent ClonesLive Confocal MicroscopyGreen Fluorescent ProteinComplex Cellular ProcessesFluorescence Video MicroscopyHuman Immunodeficiency Virus Core ProteinGag ProteinInfectious Molecular CloneHIV Gag-iGFPVideo Confocal MicroscopyTransfectHuman T Cell LineFluorescently Labeled Uninfected CD4+ T CellsTarget CellsViral ProductionTransportIntercellular StructuresVirological SynapsesGas Permeable Imaging Chambers
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Dale, B., McNerney, G. P., Thompson, D. L., Hübner, W., Huser, T., Chen, B. K. Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy. J. Vis. Exp. (44), e2061, doi:10.3791/2061 (2010).
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