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Journal
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Neuroscience
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Obtaining Single-Cell Suspensions from Murine Brain and Skull Bone Marrow
/
Preparing Bone Marrow Single-Cell Suspension from Mouse Calvaria to Study Immune Response to Brain Disorders
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Preparing Bone Marrow Single-Cell Suspension from Mouse Calvaria to Study Immune Response to Brain Disorders
Preparing Bone Marrow Single-Cell Suspension from Mouse Calvaria to Study Immune Response to Brain Disorders
Obtaining Single-Cell Suspensions from Murine Brain and Skull Bone Marrow
DOI:
10.3791/202037-v
•
01:56 min
•
July 26, 2024
•
Ran Zhang
,
Jin Zhang
,
Ata Ur Rehman
,
Lihong Dang
,
Xinyuan Yu
,
Wei Yang
1
Multidisciplinary Brain Protection Program (MBPP), Department of Anesthesiology
,
Duke University Medical Center
Tags
Bone Marrow
Single-cell Suspension
Mouse Calvaria
Immune Response
Brain Disorders
Dura Mater
PBS
Dissecting Microscope
Forceps
Pipette
Scissors
18 Gauge Needle
Microcentrifuge Tube
Centrifuge
Red Blood Cell Lysis Buffer
15 Milliliter Centrifuge Tube
Cell Pellet
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