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Obtaining Single-Cell Suspensions from Murine Brain and Skull Bone Marrow
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Preparing Bone Marrow Single-Cell Suspension from Mouse Calvaria to Study Immune Response to Brain Disorders
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JoVE Journal Neurowissenschaften
Preparing Bone Marrow Single-Cell Suspension from Mouse Calvaria to Study Immune Response to Brain Disorders

Preparing Bone Marrow Single-Cell Suspension from Mouse Calvaria to Study Immune Response to Brain Disorders

Obtaining Single-Cell Suspensions from Murine Brain and Skull Bone Marrow

DOI:
10.3791/202037-v

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01:56 min

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July 26, 2024

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, , , , ,
1Multidisciplinary Brain Protection Program (MBPP), Department of Anesthesiology,Duke University Medical Center

Tags

Bone MarrowSingle-cell SuspensionMouse CalvariaImmune ResponseBrain DisordersDura MaterPBSDissecting MicroscopeForcepsPipetteScissors18 Gauge NeedleMicrocentrifuge TubeCentrifugeRed Blood Cell Lysis Buffer15 Milliliter Centrifuge TubeCell Pellet

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