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Journal
/
Developmental Biology
/
Profiling Genomic Histone Marks in Mouse Myoblasts Using CUT&Tag
/
Genomic Purification and Library Preparation of Tagmented Mouse Myoblast DNA for Epigenetic Profiling
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Genomic Purification and Library Preparation of Tagmented Mouse Myoblast DNA for Epigenetic Profiling
Genomic Purification and Library Preparation of Tagmented Mouse Myoblast DNA for Epigenetic Profiling
Profiling Genomic Histone Marks in Mouse Myoblasts Using CUT&Tag
DOI:
10.3791/201456-v
•
02:12 min
•
March 01, 2024
•
Yuefeng Li
2
,
Xiaofen Wu
2
,
Ping Hu
2
1
Guangzhou Laboratory
,
2
The Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes
,
The Fifth Affiliated Hospital of Guangzhou Medical University
Tags
Genomic Purification
Library Preparation
Tagmentation
Mouse Myoblast
DNA
Epigenetic Profiling
Tagmentation Buffer
EDTA
SDS
Proteinase K
Phenol-chloroform
Chloroform
Ethanol
DNA Precipitation
PCR
Illumina Primers
CUT&Tag
H3K4me1
MYOD1 Gene
Enhancer Regions
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