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Profiling Genomic Histone Marks in Mouse Myoblasts Using CUT&Tag
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Genomic Purification and Library Preparation of Tagmented Mouse Myoblast DNA for Epigenetic Profiling
JoVE Journal
Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Genomic Purification and Library Preparation of Tagmented Mouse Myoblast DNA for Epigenetic Profiling

Genomic Purification and Library Preparation of Tagmented Mouse Myoblast DNA for Epigenetic Profiling

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02:12 min

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March 01, 2024

DOI:

10.3791/201456-v

DOI:
10.3791/201456-v

•

02:12 min
March 01, 2024

•

1 Views
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1Guangzhou Laboratory, 2The Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes,The Fifth Affiliated Hospital of Guangzhou Medical University

Etiketler

Genomic PurificationLibrary PreparationTagmentationMouse MyoblastDNAEpigenetic ProfilingTagmentation BufferEDTASDSProteinase KPhenol-chloroformChloroformEthanolDNA PrecipitationPCRIllumina PrimersCUT&TagH3K4me1MYOD1 GeneEnhancer Regions

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Genomic Purification and Library Preparation of Tagmented Mouse Myoblast DNA for Epigenetic Profiling. J. Vis. Exp. (Pending Publication), e201456, doi:10.3791/201456 (2024).

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