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Detection and Quantification of Single mRNA Molecules in Drosophila
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Dissectie en voorbereiding van vleugelschijven en volwassen spieren van Drosophila voor RNA-fluorescentie met één molecuul in situ hybridisatie
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Dissection and Preparation of Wing Discs and Adult Muscles from Drosophila for Single-Molecule RNA Fluorescence In Situ Hybridization
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Dissectie en voorbereiding van vleugelschijven en volwassen spieren van Drosophila voor RNA-fluorescentie met één molecuul in situ hybridisatie

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03:46 min

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September 08, 2023

DOI:

10.3791/200894-v

DOI:
10.3791/200894-v

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03:46 min
September 08, 2023

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2 Views
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1Institut de Génétique et Développement de Rennes (IGDR),UMR6290 CNRS – Université de Rennes, 2BIOSIT, UAR 3480 US 018,Université de Rennes

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DrosophilaWing DiscsAdult MusclesSingle-molecule RNA FISHDissectionParaformaldehydeEthanolPBSPBTHybridizationProbeAntibodyDAPIMounting Medium

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Leroux, E., Ammar, N., Moinet, S., Pecot, T., Boukhatmi, H. Dissection and Preparation of Wing Discs and Adult Muscles from Drosophila for Single-Molecule RNA Fluorescence In Situ Hybridization . J. Vis. Exp. (Pending Publication), e200894, doi:10.3791/200894 (2023).

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