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Detection and Quantification of Single mRNA Molecules in Drosophila
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Dissection and Preparation of Wing Discs and Adult Muscles from Drosophila for Single-Molecule RNA Fluorescence In Situ Hybridization
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Dissection and Preparation of Wing Discs and Adult Muscles from Drosophila for Single-Molecule RNA Fluorescence In Situ Hybridization

Dissection and Preparation of Wing Discs and Adult Muscles from Drosophila for Single-Molecule RNA Fluorescence In Situ Hybridization

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03:46 min

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September 08, 2023

DOI:

10.3791/200894-v

DOI:
10.3791/200894-v

•

03:46 min
September 08, 2023

•

2 Views
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1Institut de Génétique et Développement de Rennes (IGDR),UMR6290 CNRS – Université de Rennes, 2BIOSIT, UAR 3480 US 018,Université de Rennes

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DrosophilaWing DiscsAdult MusclesSingle-molecule RNA FISHDissectionParaformaldehydeEthanolPBSPBTHybridizationProbeAntibodyDAPIMounting Medium

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Leroux, E., Ammar, N., Moinet, S., Pecot, T., Boukhatmi, H. Dissection and Preparation of Wing Discs and Adult Muscles from Drosophila for Single-Molecule RNA Fluorescence In Situ Hybridization . J. Vis. Exp. (Pending Publication), e200894, doi:10.3791/200894 (2023).

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