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(充电)酰化tRNA的使用微阵列技术水平的全基因组分析
JoVE Journal
Biology
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JoVE Journal
Biology
Genome-wide Analysis of Aminoacylation (Charging) Levels of tRNA Using Microarrays
Please note that all translations are automatically generated.
Click here for the English version.
(充电)酰化tRNA的使用微阵列技术水平的全基因组分析
DOI:
10.3791/2007-v
•
07:32 min
•
June 18, 2010
•
John Zaborske
,
Tao Pan
1
Department of Biochemistry and Molecular Biology
,
University of Chicago
Chapters
00:00
Title
00:45
Introductions
01:06
Isolation of Total Charged tRNA
02:37
Cy3/Cy5 Labeling of tRNA
05:15
Hybridization and Analysis of the Microarray
06:33
Representative Results of Isolation and Analysis of tRNA
07:09
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一种用于微阵列分析的方法来确定所有的tRNA相对酰化水平<em> S。 cerivisiae。</em
Tags
Genome-wide Analysis
Aminoacylation
Charging Levels
TRNA
Microarray
Translational Regulation
Stress Response
Stringent Response
Gcn2 Stress Response Pathway
Dynamic Charging Patterns
Genomic Scale
Environmental Variations
Relative Charging Levels
S. Cerevisiae
Yeast Protocol
E. Coli
Human Cell Cultures
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