/

/

Genome-wide Analysis of Aminoacylation (Charging) Levels of tRNA Using Microarrays

Journal JoVE
Biologie

This content is Free Access.

Journal JoVE Biologie

Genome-wide Analysis of Aminoacylation (Charging) Levels of tRNA Using Microarrays

Genome-wide Analysis of Aminoacylation (Charging) Levels of tRNA Using Microarrays

DOI:

•

07:32 min

•

June 18, 2010

•

John Zaborske, Tao Pan

¹Department of Biochemistry and Molecular Biology,University of Chicago

Chapitres

00:00Titre
00:45Introductions
01:06Isolation of Total Charged tRNA
02:37Cy3/Cy5 Labeling of tRNA
05:15Hybridization and Analysis of the Microarray
06:33Representative Results of Isolation and Analysis of tRNA
07:09Conclusion

Summary

Traduction automatique

We describe a method for microarray analysis to determine relative aminoacylation levels of all tRNAs from S. cerivisiae.

Tags

Genome-wide Analysis Aminoacylation Charging Levels TRNA Microarray Translational Regulation Stress Response Stringent Response Gcn2 Stress Response Pathway Dynamic Charging Patterns Genomic Scale Environmental Variations Relative Charging Levels S. Cerevisiae Yeast Protocol E. Coli Human Cell Cultures

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Bacterial Gene Expression Analysis Using Microarrays

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans

Fabrication and Use of MicroEnvironment microArrays (MEArrays)

Glycan Profiling of Plant Cell Wall Polymers using Microarrays

A Novel Bayesian Change-point Algorithm for Genome-wide Analysis of Diverse ChIPseq Data Types

Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Read Article