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Journal
/
Immunology and Infection
/
De-Sialylated Monocyte-Derived-DCs from Isolated PBMCs Using Sialidase Treatment
/
Maturation Profiling of Human Monocyte-Derived Dendritic Cells (Mo-DCs)
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Maturation Profiling of Human Monocyte-Derived Dendritic Cells (mo-DCs)
Maturation Profiling of Human Monocyte-Derived Dendritic Cells (Mo-DCs)
De-Sialylated Monocyte-Derived-DCs from Isolated PBMCs Using Sialidase Treatment
DOI:
10.3791/200352-v
•
01:29 min
•
October 20, 2023
•
Vanessa C. C. Luz
2
,
Zélia Silva
2
,
Patrícia Sobral
2,3
,
Ankit Tanwar
5
,
Rachel L. Paterson
,
Paula A. Videira
2,7
1
Associate Laboratory i4HB – Institute for Health and Bioeconomy, NOVA School of Science and Technology
,
Universidade NOVA de Lisboa
,
2
UCIBIO – Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology
,
Universidade NOVA de Lisboa
,
3
LAQV and REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia
,
Universidade NOVA de Lisboa
,
4
Department of Microbiology and Immunology
,
Albert Einstein College of Medicine
,
5
Department of Oncology
,
Albert Einstein College of Medicine
,
6
Stemmatters, Biotecnologia e Medicina Regenerativa SA, Parque de Ciência e Tecnologia Avepark
,
Zona Industrial da Gandra
,
7
CDG & Allies – Professionals and Patient Associations International Network (CDG & Allies – PPAIN), Department of Life Sciences, NOVA School of Science and Technology
,
Universidade NOVA de Lisboa
Tags
Maturation Profiling
Monocyte-derived Dendritic Cells
Mo-DCs
Antibody Staining
Fluorescence Conjugated Antibodies
PBS
Paraformaldehyde
Flow Cytometry
MHC-I
MHC-II
CD80
CD86
Sialidase Treatment
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Maturation Profiling of Human Monocyte-Derived Dendritic Cells (Mo-DCs)
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