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De-Sialylated Monocyte-Derived-DCs from Isolated PBMCs Using Sialidase Treatment
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Maturation Profiling of Human Monocyte-Derived Dendritic Cells (Mo-DCs)
JoVE Journal
免疫学と感染
This content is Free Access.
JoVE Journal 免疫学と感染
Maturation Profiling of Human Monocyte-Derived Dendritic Cells (mo-DCs)

Maturation Profiling of Human Monocyte-Derived Dendritic Cells (Mo-DCs)

De-Sialylated Monocyte-Derived-DCs from Isolated PBMCs Using Sialidase Treatment

DOI:
10.3791/200352-v

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01:29 min

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October 20, 2023

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, , , , ,
1Associate Laboratory i4HB – Institute for Health and Bioeconomy, NOVA School of Science and Technology,Universidade NOVA de Lisboa, 2UCIBIO – Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology,Universidade NOVA de Lisboa, 3LAQV and REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia,Universidade NOVA de Lisboa, 4Department of Microbiology and Immunology,Albert Einstein College of Medicine, 5Department of Oncology,Albert Einstein College of Medicine, 6Stemmatters, Biotecnologia e Medicina Regenerativa SA, Parque de Ciência e Tecnologia Avepark,Zona Industrial da Gandra, 7CDG & Allies – Professionals and Patient Associations International Network (CDG & Allies – PPAIN), Department of Life Sciences, NOVA School of Science and Technology,Universidade NOVA de Lisboa

タグ

Maturation ProfilingMonocyte-derived Dendritic CellsMo-DCsAntibody StainingFluorescence Conjugated AntibodiesPBSParaformaldehydeFlow CytometryMHC-IMHC-IICD80CD86Sialidase Treatment

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