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Preparação de amostras, extração de RNA, limpeza por PCR e quantificação para sequenciamento do genoma completo do vírus da raiva (RABV)
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August 18, 2023
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1School of Biodiversity, One Health & Veterinary Medicine,University of Glasgow, 2Research Institute for Tropical Medicine, 3University of Nairobi, Institute of Tropical and Infectious Diseases, 4Tanzania Industrial Research Development Organization, 5Ifakara Health Institute, 6MRC-University of Glasgow Centre for Virus Research, 7Department of Veterinary Public health,Ahmadu Bello University, 8African Centre of Excellence for Neglected Tropical Diseases and Forensic Biotechnology,Ahmadu Bello University, 9University of Nairobi, Kenya and Center for Epidemiological Modelling and Analysis,University of Nairobi
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Bautista, C., Jaswant, G., French, H., Campbell, K., Durrant, R., Gifford, R., Kia, G. S. N., Ogoti, B., Hampson, K., Brunker, K. Sample Preparation, RNA Extraction, PCR Clean Up, and Quantification for Whole Genome Sequencing of Rabies Virus (RABV). J. Vis. Exp. (Pending Publication), e200317, doi:10.3791/200317 (2023).
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