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Biologie
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A Nanopore-Based Genomic Approach to Control Rabies in Low-Income Countries
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Sample Preparation, RNA Extraction, PCR Clean Up, and Quantification for Whole Genome Sequencing of Rabies Virus (RABV)
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Sample Preparation, RNA Extraction, PCR Clean Up, and Quantification for Whole Genome Sequencing of Rabies Virus (RABV)

Sample Preparation, RNA Extraction, PCR Clean Up, and Quantification for Whole Genome Sequencing of Rabies Virus (RABV)

A Nanopore-Based Genomic Approach to Control Rabies in Low-Income Countries

DOI:
10.3791/200317-v

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03:08 min

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August 18, 2023

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, , , , , , , , ,
1School of Biodiversity, One Health & Veterinary Medicine,University of Glasgow, 2Research Institute for Tropical Medicine, 3University of Nairobi, Institute of Tropical and Infectious Diseases, 4Tanzania Industrial Research Development Organization, 5Ifakara Health Institute, 6MRC-University of Glasgow Centre for Virus Research, 7Department of Veterinary Public health,Ahmadu Bello University, 8African Centre of Excellence for Neglected Tropical Diseases and Forensic Biotechnology,Ahmadu Bello University, 9University of Nairobi, Kenya and Center for Epidemiological Modelling and Analysis,University of Nairobi

Tags

Sample PreparationRNA ExtractionPCR Clean-upQuantificationWhole Genome SequencingRabies Virus (RABV)Ceramic BeadsLysis BufferBrain SampleCDNA PreparationPCR AmplificationPrimer PoolsSPRI BeadsMagnetic RackEthanol WashDNA Product

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