JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Genetics
/
A Comprehensive Approach to Studying the Promoter Interactome in Scarce Cells
/
DATP-Tailing, Adapter Ligation, and PCR Amplification of Scarce Cell DNA
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Genetics
dATP-Tailing, Adapter Ligation, and PCR Amplification of Scarce Cell DNA
DATP-Tailing, Adapter Ligation, and PCR Amplification of Scarce Cell DNA
A Comprehensive Approach to Studying the Promoter Interactome in Scarce Cells
DOI:
10.3791/200139-v
•
03:34 min
•
April 21, 2023
•
Llorenç Rovirosa*
1
,
Laureano Tomás-Daza*
1,2
,
Blanca Urmeneta
,
Alfonso Valencia
3
,
Biola M. Javierre
4
1
Josep Carreras Leukaemia Research Institute
,
2
Barcelona Supercomputing Center
,
3
Catalan Institution for Research and Advanced Studies (ICREA)
,
4
Institute for Health Science Research Germans Trias i Pujol
Tags
DATP-Tailing
Adapter Ligation
PCR Amplification
Scarce Cell DNA
Biotin
Klenow Exo-minus
Ligation Buffer
T4 DNA Ligase
Paramagnetic Bead Purification
Double-Sided Selection
TLE Buffer
70% Ethanol
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
Lysis and Digestion of Scarce Cells
Ligation and Decrosslinking of Scarce Cell DNA
Sonication and Biotin Pull-Down of Scarce Cells DNA
DATP-Tailing, Adapter Ligation, and PCR Amplification of Scarce Cell DNA
Library Capture, Biotin Pull-Down, and PCR Amplification Using Scarce Cell DNA Library
Read Article