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Genetik
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A Comprehensive Approach to Studying the Promoter Interactome in Scarce Cells
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DATP-Tailing, Adapter Ligation, and PCR Amplification of Scarce Cell DNA
JoVE Journal
Genetik
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JoVE Journal Genetik
dATP-Tailing, Adapter Ligation, and PCR Amplification of Scarce Cell DNA

DATP-Tailing, Adapter Ligation, and PCR Amplification of Scarce Cell DNA

A Comprehensive Approach to Studying the Promoter Interactome in Scarce Cells

DOI:
10.3791/200139-v

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03:34 min

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April 21, 2023

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, , , ,
1Josep Carreras Leukaemia Research Institute, 2Barcelona Supercomputing Center, 3Catalan Institution for Research and Advanced Studies (ICREA), 4Institute for Health Science Research Germans Trias i Pujol

Tags

DATP-TailingAdapter LigationPCR AmplificationScarce Cell DNABiotinKlenow Exo-minusLigation BufferT4 DNA LigaseParamagnetic Bead PurificationDouble-Sided SelectionTLE Buffer70% Ethanol

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